Recent capillary electrophoresis applications for upstream and downstream biopharmaceutical process monitoring

The biopharmaceutical market is one of the fastest growing biotechnology markets. In order to ensure affordable and reliable therapeutics, process has be closely monitored. Capillary electrophoresis (CE) proven a valuable technique for analysis product concentration, critical quality attributes, process-related impurities, nutrients metabolites in cell culture medium. zone electrophoresis, capillary gel isoelectric focusing are extensively used size or charge heterogeneity determination. CE number benefits upstream downstream process-intermediates, including ability handle highly complex matrices found high resolving power, little sample preparation requirements, rapid analysis, low solvent consumption. small volumes (nL range) especially beneficial microbioreactor clone selection experiments. simple setup possibility miniaturisation automation using microchip provides great opportunities on-site, real-time monitoring process. This review discusses applications processing last decade.